Dr Yuqin Wang

BSc, PhD

Lecturer in Mass Spectrometry

Tel:  +44 (0)1792 602730
Email: y.wang@swansea.ac.uk

Areas of Expertise

Mass Spectrometry, Proteomics, Metabolomics, Analytical Biochemistry, Lipid Metabolism, Steroids, and Receptor-Ligand Interaction.

Education

Ph.D., Biochemistry, Karolinska Institutet, Stockholm, Sweden (2001)
B.Sc., Pharmacy, Beijing Medical University, Beijing, China (1994)

Research Interests

I started my research career at Karolinska Institutet in Stockholm. At Karolinska I was
classically trained as a protein/lipid biochemist, becoming well versed in protein/lipid
purification and analysis techniques. I am particularly interested in protein-lipid
interactions and investigating the function of the bioactive lipids, such as oxysterols.
My current research concentrates on how oxysterols stimulate ERK and Sonic
Hedgehog signalling pathways and their effect on neurons.

Collaborators:

Queen Mary, University of London

GSK

Selected Publications

  1. Wang Y, Sousa KM, Bodin K, Theofilopoulos S, Sacchetti P, Hornshaw M, Woffendin G,
    Karu K, Sjövall J, Arenas E and Griffiths WJ. (2009) Targeted lipidomic analysis of
    oxysterols in the embryonic central nervous system. Molecular BioSystems,
    DOI:10.1039/B819502A

  2. Griffiths, W. J. & Wang, Y. (2009). The importance of steroidomics in the study of
    neurodegenerative disease and ageing. Comb. Chem. High Throughput. Screen. 12,
    212-228.

  3. Wang Y, Griffiths WJ. (2008) Mass Spectrometry: From Proteomics to Metabolomics and
    Lipidomics. Chemical Society Reviews, DOI:10.1039/B618553N

  4. Seibert C, Davidson BR, Fuller BJ, Patterson LH, Griffiths WJ, Wang Y. Multiple-
    Approaches to the Identification and Quantification of Cytochromes P450 in Human Liver
    Tissue by Mass Spectrometry. J Proteome Res. (2008) Dec 29. [Epub ahead of print]

  5. Wang Y, Mulligan C, Denyer G, Delom F, gna-Bricarelli F, Tybulewicz VL, Fisher EM,
    Griffiths WJ, Nizetic D, and Groet J. Quantitative proteomic characterisation of a mouse
    embryonic stem cell model of Down syndrome. Mol. Cell Proteomics. (2008) Nov 18.
    [Epub ahead of print]

  6. Griffiths, W. J., Wang, Y., Karu, K., Samuel, E., McDonnell, S., Hornshaw, M. &
    Shackleton, C. (2008). Potential of sterol analysis by liquid chromatography-tandem
    mass spectrometry for the prenatal diagnosis of Smith-Lemli-Opitz syndrome. Clin.
    Chem.     54, 1317-1324.

  7. Griffiths, W. J., Hornshaw, M., Woffendin, G., Baker, S. F., Lockhart, A., Heidelberger, S.,
    Gustafsson, M., Sjovall, J. & Wang, Y. (2008). Discovering oxysterols in plasma: a
    window on the metabolome. J. Proteome. Res. 7, 3602-3612.

  8. Wang, Y., Muneton, S., Sjovall, J., Jovanovic, J. N. & Griffiths, W. J. (2008). The effect of
    24S-hydroxycholesterol on cholesterol homeostasis in neurons: quantitative changes to
    the cortical neuron proteome. J. Proteome. Res. 7, 1606-1614.

  9. Wang, Y. & Griffiths, W. J. (2008). Capillary liquid chromatography combined with
    tandem mass spectrometry for the study of neurosteroids and oxysterols in brain.
    Neurochem. Int. 52, 506-521.

  10. Griffiths, W. J., Karu, K., Hornshaw, M., Woffendin, G. & Wang, Y. (2007). Metabolomics
    and metabolite profiling: past heroes and future developments. Eur. J. Mass Spectrom.
    (Chichester, Eng) 13, 45-50.

  11. Karu, K., Hornshaw, M., Woffendin, G., Bodin, K., Hamberg, M., Alvelius, G., Sjovall, J.,
    Turton, J., Wang, Y. & Griffiths, W. J. (2007). Liquid chromatography-mass spectrometry
    utilizing multi-stage fragmentation for the identification of oxysterols. J. Lipid Res. 48,
    976-987.

  12. Lane, C. S., Wang, Y., Betts, R., Griffiths, W. J. & Patterson, L. H. (2007). Comparative
    cytochrome P450 proteomics in the livers of immunodeficient mice using 18O stable
    isotope labeling. Mol. Cell Proteomics. 6, 953-962.

  13. Wang, Y., Karu, K. & Griffiths, W. J. (2007). Analysis of neurosterols and neurosteroids
    by mass spectrometry. Biochimie 89, 182-191.

  14. Griffiths, W. J., Wang, Y., Alvelius, G., Liu, S., Bodin, K. & Sjovall, J. (2006). Analysis of
    oxysterols by electrospray tandem mass spectrometry. J. Am. Soc. Mass Spectrom. 17,
    341-362.

  15. Khan, M. A., Wang, Y., Heidelberger, S., Alvelius, G., Liu, S., Sjovall, J. & Griffiths, W. J.
    (2006). Analysis of derivatised steroids by matrix-assisted laser desorption/ionisation and
    post-source decay mass spectrometry. Steroids 71, 42-53.

  16. Wang, Y., Al-Gazzar, A., Seibert, C., Sharif, A., Lane, C. & Griffiths, W. J. (2006).
    Proteomic analysis of cytochromes P450: a mass spectrometry approach. Biochem. Soc.
    Trans.     34, 1246-1251.

  17. Wang, Y., Hornshaw, M., Alvelius, G., Bodin, K., Liu, S., Sjovall, J. & Griffiths, W. J.
    (2006). Matrix-assisted laser desorption/ionization high-energy collision-induced
    dissociation of steroids: analysis of oxysterols in rat brain. Anal. Chem. 78, 164-173.

  18. Heap, C. J., Wang, Y., Pinheiro, T. J., Reading, S. A., Jennings, K. R. & Dimmock, N. J.
    (2005). Analysis of a 17-amino acid residue, virus-neutralizing microantibody. J. Gen.
    Virol.     86, 1791-1800.

  19. Wang, Y., Walter, G., Herting, E., Agerberth, B. & Johansson, J. (2004). Antibacterial
    activities of the cathelicidins prophenin (residues 62 to 79) and LL-37 in the presence of a
    lung surfactant preparation. Antimicrob. Agents Chemother. 48, 2097-2100.

  20. Wang, Y., Johansson, J., Agerberth, B., Jornvall, H. & Griffiths, W. J. (2004). The
    antimicrobial peptide LL-37 binds to the human plasma protein apolipoprotein A-I. Rapid
    Commun. Mass Spectrom.     18, 588-589.

  21. Wang, Y., Griffiths, W. J., Jornvall, H., Agerberth, B. & Johansson, J. (2002). Antibacterial
    peptides in stimulated human granulocytes: characterization of ubiquitinated histone
    H1A. Eur. J. Biochem. 269, 512-518.

  22. Griffiths, W. J., Jonsson, A. P., Liu, S., Rai, D. K. & Wang, Y. (2001). Electrospray and
    tandem mass spectrometry in biochemistry. Biochem. J. 355, 545-561.

  23. Wang, Y., Johansson, J. & Griffiths, W. J. (2000). Characterisation of variant forms of
    prophenin: mechanistic aspects of the fragmentation of proline-rich peptides. Rapid
    Commun. Mass Spectrom.     14, 2182-2202.

  24. Wang, Y., Griffiths, W. J., Curstedt, T. & Johansson, J. (1999). Porcine pulmonary
    surfactant preparations contain the antibacterial peptide prophenin and a C-terminal 18-
    residue fragment thereof. FEBS Lett. 460, 257-262.

  25. Stark, M., Wang, Y., Danielsson, O., Jornvall, H. & Johansson, J. (1998). Determination
    of proteins, phosphatidylethanolamine, and phosphatidylserine in organic solvent extracts
    of tissue material by analysis of phenylthiocarbamyl derivatives. Anal. Biochem. 265, 97-
    102.

  26. Wang, Y., Agerberth, B., Lothgren, A., Almstedt, A. & Johansson, J. (1998).
    Apolipoprotein A-I binds and inhibits the human antibacterial/cytotoxic peptide LL-37. J.
    Biol. Chem.     273, 33115-33118.

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