Professor William Griffiths

The Griffiths – Wang research group is focused on the mass spectrometric analysis of lipids and proteins. Current projects involve the study of cholesterol biosynthesis and metabolism, particularly the formation of oxysterols and bile acids. New and improved methods for the identification, quantification, and characterization of bile acids, oxysterols, and other sterols and steroids, are being developed; and the involvement of these molecules in neurogenesis and immunity is being investigated. Complementary to this, proteomic techniques are being used to study biosynthetic and metabolizing enzymes in tissues and cells. This research fits within the College Biomarker & Genes, and Microbes & Immunity themes.

Publications

  1. et. al. International descriptive and interventional survey for oxycholesterol determination by gas- and liquid-chromatographic methods. Biochimie 153, 26-32.
  2. & Identification of 7α,24-dihydroxy-3-oxocholest-4-en-26-oic and 7α,25-dihydroxy-3-oxocholest-4-en-26-oic acids in human cerebrospinal fluid and plasma. Biochimie
  3. & Identification Of Unusual Oxysterols And Bile acids With 7-Oxo Or 3β,5α,6β-Trihydroxy Functions In Human Plasma By Charge-Tagging Mass Spectrometry With Multistage Fragmentation. Journal of Lipid Research 59(4), jlr.D083246
  4. & Modulation of Kv3.1b potassium channel level and intracellular potassium concentration in 158N murine oligodendrocytes and BV-2 murine microglial cells treated with 7-ketocholesterol, 24S-hydroxycholesterol or tetracosanoic acid (C24:0). Biochimie
  5. & An update on oxysterol biochemistry: New discoveries in lipidomics. Biochemical and Biophysical Research Communications 504(3), 617-622.

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Teaching

  • PM-304 Biomolecular Research Project

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  • PMLM06 LC/MS Applications V: Metabolomics, Lipidomics and Bioactive lipids

    This module covers the basic concepts of metabolomics, lipidomics and analysis of bioactive lipids. The module will focus on the application of LC-MS analysis of biomolecules isolated from complex mixtures.

  • PMLM07 Data analysis and method development

    This module will allow students to statistically evaluate their data acquired using LC-MS to provide meaningful conclusions for their work. This will involve the practical use of `state-of-the-art¿ databases and software analysis tools available to the mass spectrometry community. Students will gain experience using the latest software technology and techniques for their future careers.

  • PMLM09 Dissertation: MS experimental project.

    Students will have the opportunity to join an established research group to work in a modern and well equipped laboratory or alternatively undertake a suitable non-laboratory based project. In this module the student will be able to gain extensive `hands on¿ specialist expertise in a chosen topic which could be targeted to their future career in the field of mass spectrometry.

Supervision

  • Sensing bacterial infection: 25-hydroxycholesterol as a biomarker (current)

    Student name:
    PhD
    Other supervisor: Prof Yuqin Wang
    Other supervisor: Prof Thomas Humphrey
    Other supervisor: Dr Thomas Wilkinson
  • Untitled (current)

    Student name:
    PhD
    Other supervisor: Dr Owain Howell
  • «br /»«br /»«br /»«br /»«br /»«br /»«br /»«br /»«br /»«br /»«br /»«br /»«br /»«br /» Development and Validation of Assays for Vitamins D and other Sterols using LC-MS and GC-TOF-MS with select -eV (current)

    Student name:
    PhD
    Other supervisor: Prof Yuqin Wang
  • Inhibiting IL-17 Production by Blocking Endogenous RORγt Agonists for the Treatment of Autoimmune Diseases (current)

    Student name:
    PhD
    Other supervisor: Prof Catherine Thornton
    Other supervisor: Dr Jonathan Mullins
    Other supervisor: Prof Yuqin Wang
  • 'Development of mass spectrometry based reference methods for the measurement of 25-hydroxyvitamins D2 and D3 and the discovery of related molecules in human serum' (awarded 2017)

    Student name:
    PhD
    Other supervisor: Prof Yuqin Wang
  • 'Lipidomic analysis of sterols in Human T Helper cells' (awarded 2017)

    Student name:
    PhD
    Other supervisor: Prof Yuqin Wang